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‒ anti cd137 pe vio 615  (Miltenyi Biotec)


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    Miltenyi Biotec ‒ anti cd137 pe vio 615
    ‒ Anti Cd137 Pe Vio 615, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd137/CD4+Antibody%2C+anti-mouse%2C+REAfinity/pm42098128-440-36-41
    Average 95 stars, based on 122 article reviews
    ‒ anti cd137 pe vio 615 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Magnetic Cell Separation:

    Article Title: Generation of T cell responses against broad KRAS hotspot neoantigens for cell therapy or TCR discovery.
    Article Snippet: Samples were resuspended in autoMACS Rinsing Solution with MACS BSA Stock Solution and positively enriched for either CD137+ (Miltenyi) or CD69+ cells (Miltenyi) following the manufacturer’s instructions at a cell density of less than 2x108 labeled cells per LS column with a 30μm cell strainer. .. Samples were resuspended in autoMACS Rinsing Solution with MACS BSA Stock Solution and positively enriched for either CD137+ (Miltenyi) or CD69+ cells (Miltenyi) following the manufacturer’s instructions at a cell density of less than 2x108 labeled cells per LS column with a 30μm cell strainer. .. Enriched cells were cultured for between 6 and 14 days at a seeding density of 5x105/cm2 in a G-Rex-24 Well in AIM-V + 5% HS, 5 ng/mL of IL-7 and 5 ng/mL of IL-15, and between 10 and 300 U/mL IL-2 (CellGenix).

    Article Title: Generation of T cell responses against broad KRAS hotspot neoantigens for cell therapy or TCR discovery
    Article Snippet: .. Samples were resuspended in autoMACS Rinsing Solution with MACS BSA Stock Solution and positively enriched for either CD137 + (Miltenyi) or CD69 + cells (Miltenyi) following the manufacturer’s instructions at a cell density of less than 2x10 8 labeled cells per LS column with a 30μm cell strainer. .. Enriched cells were cultured for between 6 and 14 days at a seeding density of 5x10 5 /cm 2 in a G-Rex-24 Well in AIM-V + 5% HS, 5 ng/mL of IL-7 and 5 ng/mL of IL-15, and between 10 and 300 U/mL IL-2 (CellGenix).

    Labeling:

    Article Title: Generation of T cell responses against broad KRAS hotspot neoantigens for cell therapy or TCR discovery.
    Article Snippet: Samples were resuspended in autoMACS Rinsing Solution with MACS BSA Stock Solution and positively enriched for either CD137+ (Miltenyi) or CD69+ cells (Miltenyi) following the manufacturer’s instructions at a cell density of less than 2x108 labeled cells per LS column with a 30μm cell strainer. .. Samples were resuspended in autoMACS Rinsing Solution with MACS BSA Stock Solution and positively enriched for either CD137+ (Miltenyi) or CD69+ cells (Miltenyi) following the manufacturer’s instructions at a cell density of less than 2x108 labeled cells per LS column with a 30μm cell strainer. .. Enriched cells were cultured for between 6 and 14 days at a seeding density of 5x105/cm2 in a G-Rex-24 Well in AIM-V + 5% HS, 5 ng/mL of IL-7 and 5 ng/mL of IL-15, and between 10 and 300 U/mL IL-2 (CellGenix).

    Article Title: Generation of T cell responses against broad KRAS hotspot neoantigens for cell therapy or TCR discovery
    Article Snippet: .. Samples were resuspended in autoMACS Rinsing Solution with MACS BSA Stock Solution and positively enriched for either CD137 + (Miltenyi) or CD69 + cells (Miltenyi) following the manufacturer’s instructions at a cell density of less than 2x10 8 labeled cells per LS column with a 30μm cell strainer. .. Enriched cells were cultured for between 6 and 14 days at a seeding density of 5x10 5 /cm 2 in a G-Rex-24 Well in AIM-V + 5% HS, 5 ng/mL of IL-7 and 5 ng/mL of IL-15, and between 10 and 300 U/mL IL-2 (CellGenix).

    Isolation:

    Article Title: Proteogenomic analysis reveals RNA as a source for tumor-agnostic neoantigen identification.
    Article Snippet: .. CD137-expressing activated cells were isolated andenrichedusing thehumanCD137MicroBeadKit (Ref. 130-093-476, Miltenyi) according to the manufacturer’s instructions. .. Enriched cells were taken into a culture in T cell medium (TCM, see Methods—primary human material and cell lines) supplemented with 5 ng/mL IL-7 (Ref. 200-07, Peprotech), 5 ng/mL IL-15 (Ref. 200-15, Peprotech), 30U/mL IL-2 (Ref. 200-02, Peprotech), and 30ng/mL OKT-3 (kindly provided by Elisabeth Kremmer) along with 1 Mio irradiated (30 Gray) feeder PBMC.

    Incubation:

    Article Title: Inherited human CARD9 deficiency impairs lymphoid cell, but not fibroblast, IL-17–mediated immunity
    Article Snippet: Brefeldin A (B7651-5MG, Sigma Aldrich, 10 mg/mL) was added to the culture for the last 4 hours of incubation. .. Harvested cells were incubated with conjugated mAbs directed against CD4 (BUV737, clone SK3, 612748, BD Horizon), CD8 (BUV395, clone RPA-T8, 563795, BD Horizon), CD25 (FITC, clone 2A3, 347643, BD Biosciences), CD45RA (PerCPCy5.5, clone HI100, 45-0458-42, eBioscience), CCR7/CD197(PECy7, clone G043H7, 353226, BioLegend), CD137 (APC, clone REA765, 130-110-764, Miltenyi Biotec), and OX40 (PE, clone ACT35, 12-1347-42, eBioscience). .. For the assessment of intracellular cytokine production, cells were incubated with conjugated mAbs directed against IFN-γ (BUV737, clone 4S.B3, 564620, BD Horizon), TNF-α (PerCP, clone Mab11, 502924, BioLegend), IL-17A (BV510, clone BL168, 512330, BioLegend), IL-17F (BV650, clone O33-782, 562264, BD Horizon), and IL-2 (BV750, clone MQ1-17H12, 566361, BD Horizon).

    Article Title: Inherited human CARD9 deficiency impairs lymphoid cell, but not fibroblast, IL-17-mediated immunity.
    Article Snippet: Brefeldin A (B7651-5MG, Sigma Aldrich, 10 mg/mL) was added to the culture for the last 4 hours of incubation. .. Harvested cells were incubated with conjugated mAbs directed against CD4 (BUV737, clone SK3, 612748, BD Horizon), CD8 (BUV395, clone RPA-T8, 563795, BD Horizon), CD25 (FITC, clone 2A3, 347643, BD Biosciences), CD45RA (PerCPCy5.5, clone HI100, 45-0458-42, eBioscience), CCR7/CD197(PECy7, clone G043H7, 353226, BioLegend), CD137 (APC, clone REA765, 130-110-764, Miltenyi Biotec), and OX40 (PE, clone ACT35, 12-1347-42, eBioscience). .. For the assessment of intracellular cytokine production, cells were incubated with conjugated mAbs directed against IFN-γ (BUV737, clone 4S.B3, 564620, BD Horizon), TNF-α (PerCP, clone Mab11, 502924, BioLegend), IL-17A (BV510, clone BL168, 512330, BioLegend), IL-17F (BV650, clone O33-782, 562264, BD Horizon), and IL-2 (BV750, clone MQ1-17H12, 566361, BD Horizon).

    Recombinase Polymerase Amplification:

    Article Title: Inherited human CARD9 deficiency impairs lymphoid cell, but not fibroblast, IL-17–mediated immunity
    Article Snippet: Brefeldin A (B7651-5MG, Sigma Aldrich, 10 mg/mL) was added to the culture for the last 4 hours of incubation. .. Harvested cells were incubated with conjugated mAbs directed against CD4 (BUV737, clone SK3, 612748, BD Horizon), CD8 (BUV395, clone RPA-T8, 563795, BD Horizon), CD25 (FITC, clone 2A3, 347643, BD Biosciences), CD45RA (PerCPCy5.5, clone HI100, 45-0458-42, eBioscience), CCR7/CD197(PECy7, clone G043H7, 353226, BioLegend), CD137 (APC, clone REA765, 130-110-764, Miltenyi Biotec), and OX40 (PE, clone ACT35, 12-1347-42, eBioscience). .. For the assessment of intracellular cytokine production, cells were incubated with conjugated mAbs directed against IFN-γ (BUV737, clone 4S.B3, 564620, BD Horizon), TNF-α (PerCP, clone Mab11, 502924, BioLegend), IL-17A (BV510, clone BL168, 512330, BioLegend), IL-17F (BV650, clone O33-782, 562264, BD Horizon), and IL-2 (BV750, clone MQ1-17H12, 566361, BD Horizon).

    Article Title: Inherited human CARD9 deficiency impairs lymphoid cell, but not fibroblast, IL-17-mediated immunity.
    Article Snippet: Brefeldin A (B7651-5MG, Sigma Aldrich, 10 mg/mL) was added to the culture for the last 4 hours of incubation. .. Harvested cells were incubated with conjugated mAbs directed against CD4 (BUV737, clone SK3, 612748, BD Horizon), CD8 (BUV395, clone RPA-T8, 563795, BD Horizon), CD25 (FITC, clone 2A3, 347643, BD Biosciences), CD45RA (PerCPCy5.5, clone HI100, 45-0458-42, eBioscience), CCR7/CD197(PECy7, clone G043H7, 353226, BioLegend), CD137 (APC, clone REA765, 130-110-764, Miltenyi Biotec), and OX40 (PE, clone ACT35, 12-1347-42, eBioscience). .. For the assessment of intracellular cytokine production, cells were incubated with conjugated mAbs directed against IFN-γ (BUV737, clone 4S.B3, 564620, BD Horizon), TNF-α (PerCP, clone Mab11, 502924, BioLegend), IL-17A (BV510, clone BL168, 512330, BioLegend), IL-17F (BV650, clone O33-782, 562264, BD Horizon), and IL-2 (BV750, clone MQ1-17H12, 566361, BD Horizon).

    Staining:

    Article Title: Blinatumomab bypasses CD28 blockade to sustain T-cell cytotoxicity and improve survival in a xenograft B-ALL model
    Article Snippet: Cells were incubated for an additional 2.5 hours, centrifuged, and resuspended in PBS (Cytiva). .. Surface staining was performed for 30 min using the following antibodies: CD25 fluorescein isothiocyanate (BioLegend Cat# 356106), CD3 BV421 (BioLegend Cat# 317332), CD4 APC-Cy7 (BioLegend Cat# 317418), CD8 BV785 (BioLegend Cat# 301046), CD137 (Miltenyi Cat# 130-110-763), CD69 PE-Texas Red (BioLegend Cat# 310942), OX40 UV575 (Miltenyi Cat# 130-120-723), and CD56 PE-Cy5 (BioLegend Cat# 362516). .. Subsequently, cells were fixed and permeabilized (Invitrogen), followed by intracellular staining with antihuman IFN-γ Alexa Fluor 670 (BioLegend Cat# 502516) for 30 min on ice in the dark.

    Article Title: Magnitude and dynamics of the T-cell response to SARS-CoV-2 infection at both individual and population levels.
    Article Snippet: .. At 18 hours, cells were harvested and stained with antibodies for magnetic enrichment based on CD137 (Miltenyi Biotec, Gaithersburg, MD) and subsequent sorting by flow cytometry. ..

    Article Title: Magnitude and dynamics of the T-cell response to SARS-CoV-2 infection at both individual and population levels
    Article Snippet: .. At 18 hours, cells were harvested and stained with antibodies for magnetic enrichment based on CD137 (Miltenyi Biotec, Gaithersburg, MD) and subsequent sorting by flow cytometry. ..

    Flow Cytometry:

    Article Title: Magnitude and dynamics of the T-cell response to SARS-CoV-2 infection at both individual and population levels.
    Article Snippet: .. At 18 hours, cells were harvested and stained with antibodies for magnetic enrichment based on CD137 (Miltenyi Biotec, Gaithersburg, MD) and subsequent sorting by flow cytometry. ..

    Article Title: Magnitude and dynamics of the T-cell response to SARS-CoV-2 infection at both individual and population levels
    Article Snippet: .. At 18 hours, cells were harvested and stained with antibodies for magnetic enrichment based on CD137 (Miltenyi Biotec, Gaithersburg, MD) and subsequent sorting by flow cytometry. ..



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